Specific labeling of cytosolic and mitochondrial aspartate aminotransferases
نویسندگان
چکیده
منابع مشابه
Changes in the Level of Mitochondrial and Cytosolic Aspartate Aminotransferase Activities in Aluminium Intoxified Rat
The activity of aspartate aminotransferase (AST) in human serum has been widely determined as a diagnostic aid in liver disease. In this study, the effect of aluminium on AST isoenzymes in relation to aluminium intoxified patients has been investigated. Using gel filtration chromatography technique with Sephacryl S-300, mitochondrial aminotransferase (m-AST) and cytosolic aminotransferase (c-AS...
متن کاملPrimary structures of aspartate aminotransferases.
three residues seems to be common to all the serine enzymes and has become known as the ‘charge-relay system’. Its function is to transfer a negative (or partial negative) charge from the aspartic acid residue to the oxygen atom of the hydroxy group of serine. This obviously increases the nucleophilicity of the serine, but the important point is that the charge is developed in the absence of bu...
متن کاملchanges in the level of mitochondrial and cytosolic aspartate aminotransferase activities in aluminium intoxified rat
the activity of aspartate aminotransferase (ast) in human serum has been widely determined as a diagnostic aid in liver disease. in this study, the effect of aluminium on ast isoenzymes in relation to aluminium intoxified patients has been investigated. using gel filtration chromatography technique with sephacryl s-300, mitochondrial aminotransferase (m-ast) and cytosolic aminotransferase (c-as...
متن کاملComparison of specific and relative alanine and aspartate aminotransferases of Trypanosoma brucei subgroup trypanosomes.
Using Biochemica Test Kits (Boehringer GmbH, Mannheim) a significant difference in alanine aminotransferase : aspartate aminotransferase (ALAT : ASAT) ratios was found between Trypanosoma brucei brucei (4 strains; mean ± S.E.; 4.204 ± 0.342) and T. b. rhodesiense (4 strains; mean ± S.E.: 2.973 ± 0.174) blood¬ stream stage crude soluble enzyme extracts. This difference is a stable characteristic...
متن کاملIdentification of an adipocyte-specific negative glucose response region in the cytosolic aspartate aminotransferase gene.
Cytosolic aspartate aminotransferase (cAspAT) participates in gluconeogenesis in the liver and is expected to exert a glyceroneogenic function in the adipose tissue when the supply of glucose is limited. Here we demonstrate that adipose cAspAT messenger RNA (mRNA) is increased when rats are fed a low carbohydrate diet. In the 3T3-F442A, BFC-1 adipocyte cell lines and differentiated adipocytes i...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1985
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1985.tb08696.x